The influence of nanomaterials on pyocyanin production by Pseudomonas aeruginosa

Abstract Pseudomonas aeruginosa is a bacterium producing industrially utile metabolites, such as rhamnolipids, biopolymers, and pigments. Pyocyanin, the most studied example of pigments, virulence factor that also shows potential for application in, e.g., agriculture, anticancer therapy, energy production. Therefore, inhibitors stimulants pyocyanin production by P. should be studied, nanomaterials may cause both effects. The study aimed to examine influence zinc oxide multi-walled carbon nanotubes (pristine or dispersed with alginic acid) on . First, different concentrations (500.00–0.06 µg/mL) culture optical density biofilm formation was studied. These results helped select further tests, i.e., growth curves fluorescence measurements. Pyocyanin assessed chloroform–hydrochloric acid method. SEM analysis conducted assess cell's integrity structure. Pristine exhibited stimulative effect pigment when applied in high (500.00 µg/mL), while material enhanced lowered dosages (125.00 µg/mL). On other hand, inhibited production, minor increased bioproduct research indicates use modulators metabolites.